| Sat 20/08/16 | Irlam v Barnoldswick Town * | 1-0 | | | | |
| Sat 27/08/16 | Barnoldswick Town v New Mills | 4-2 | | | | |
| Tue 30/08/16 | Nelson v Barnoldswick Town | 1-2 | | | | |
| Sat 03/09/16 | Barnoldswick Town v 1874 Northwich | 2-2 | | | | |
| Mon 05/09/16 | Atherton Collieries v Barnoldswick Town | 3-0 | | | | |
| Sat 10/09/16 | Darlington Railway Athletic v Barnoldswick Town | 0-4 | | | | |
| Sat 17/09/16 | Barnoldswick Town v Runcorn Linnets | 0-2 | |  | | |
| Tue 20/09/16 | Squires Gate v Barnoldswick Town | 2-3 | | | | |
| Sat 24/09/16 | Barnoldswick Town v Stokesley Sports Club | 7-1 | | | | |
| Tue 27/09/16 | Barnoldswick Town v Irlam | 3-0 | | | | |
| Sat 01/10/16 | West Didsbury & Chorlton v Barnoldswick Town | 1-3 | | | | |
| Tue 04/10/16 | Barnoldswick Town v Padiham | 1-2 | | | | |
| Sat 08/10/16 | Barnoldswick Town v Bootle | 1-2 | | | | |
| Tue 11/10/16 | Barnoldswick Town v Squires Gate | 2-4 |  | | | |
| Sat 15/10/16 | Winsford United v Barnoldswick Town | 2-1 | | | | |
| Sat 22/10/16 | Charnock Richard v Barnoldswick Town | 0-0 | | | | |
| Sat 29/10/16 | Barnoldswick Town v Cammell Laird 1907 | 2-1 | | | | |
| Tue 01/11/16 | Barnoldswick Town v AFC Darwen | 1-1 | | | | |
| Sat 05/11/16 | Cammell Laird 1907 v Barnoldswick Town | 1-1 | | | | |
| Tue 08/11/16 | Harrogate Town v Barnoldswick Town | 4-0 | | | | |
| Sat 03/12/16 | Barnoldswick Town v Cammell Laird 1907 | 2-0 | | | | |
| Tue 06/12/16 | Barnoldswick Town v Abbey Hey | 0-0 | | | | |
| Sat 10/12/16 | Abbey Hey v Barnoldswick Town | 2-0 | | | | |
| Sat 17/12/16 | Barnoldswick Town v Ashton Athletic | 0-0 | | | | |
| Sat 31/12/16 | Ashton Athletic v Barnoldswick Town | 0-4 | | | | |
| Sat 07/01/17 | Barnoldswick Town v Maine Road | 1-1 | | | | |
| Sat 21/01/17 | Runcorn Linnets v Barnoldswick Town | 2-0 | | | | |
| Sat 04/02/17 | 1874 Northwich v Barnoldswick Town | 2-0 | | | | |
| Sat 18/02/17 | Congleton Town v Barnoldswick Town | 1-1 | | | | |
| Tue 07/03/17 | Barnoldswick Town v Abbey Hey | 3-0 | | | | |
| Sat 11/03/17 | Widnes v Barnoldswick Town | 0-1 | | | | |
| Tue 14/03/17 | Barnoldswick Town v Nelson | 2-1 | | | | |
| Fri 17/03/17 | Runcorn Town v Barnoldswick Town | 3-4 | | | | |
| Sat 25/03/17 | Barnoldswick Town v Congleton Town | 3-3 | | | | |
| Tue 28/03/17 | West Didsbury & Chorlton v Barnoldswick Town | 3-3 | | | | |
| Sat 01/04/17 | Barnoldswick Town v Hanley Town | 2-1 | | | | |
| Tue 04/04/17 | Barnoldswick Town v Atherton Collieries | 1-2 | | | | |
| Thu 06/04/17 | Barnoldswick Town v Bootle | 1-0 | | | | |
| Sat 08/04/17 | Barnton v Barnoldswick Town * | 1-0 | | | | |
| Tue 11/04/17 | Bootle v Barnoldswick Town | 6-1 | | | | |
| Thu 13/04/17 | Bootle v Barnoldswick Town | 2-3 | | | | |
| Sat 15/04/17 | Barnoldswick Town v West Didsbury & Chorlton | 1-3 | | | | |
| Mon 17/04/17 | New Mills v Barnoldswick Town | 3-0 | | | | |
| Sat 22/04/17 | Hanley Town v Barnoldswick Town | 2-3 | | | | |
| Mon 24/04/17 | Barnoldswick Town v Runcorn Town | 7-2 | | | | |
| Tue 25/04/17 | Barnoldswick Town v Barnton | 0-2 | | | | |
| Thu 27/04/17 | Barnoldswick Town v AFC Liverpool | 5-2 | | | | |
| Sat 29/04/17 | Barnoldswick Town v Winsford United | 2-1 | | | | |
| Tue 16/05/17 | Barnoldswick Town v City of Liverpool FC | 1-1 | | | | |
